产品目录

分子量：Approximately 21.1kDa,a single non-glycosylated polypeptide chain containing 191 amino acids.
储存条件：冷冻（-20℃）

概述

TNF RI,also named as TNFR-I,TNF R1,TNFRSF1A,p55 and p60,belongs to the tumor necrosis factor receptor superfamily and it is encoded by the TNFRSF1A gene in human.TNF RI is a universally expressed protein and is thought to form homotrimers after ligand binding.TNF RI is one of the major receptors for the TNF-α.This receptor can activate the transcription factor NF-κB,mediate apoptosis,and function as a regulator of inflammation.Murine TNF RI shares 70%,88%,67%,70%and 64%a.a.sequence identity with human,rat,canine,feline and porcine TNF RI,respectively.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞97%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
生物活性	Fully biologically active when compared to standard.The ED50 as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than 1μg/ml,corresponding to a specific activity of>1000 IU/mg in the presence of 0.1ng/mL of rMuTNF-α.
成分	Lyophilized from a 0.2μmfiltered solution in PBS,pH 7.4.
内毒素	Less than 0.1 EU/μg of rMusTNF RI/TNFRSF1A as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	IHPSGVTGLV PSLGDREKRD SLCPQGKYVH SKNNSICCTK CHKGTYLVSD CPSPGRDTVC RECEKGTFTA SQNYLRQCLS CKTCRKEMSQ VEISPCQADK DTVCGCKENQ FQRYLSETHF QCVDCSPCFN GTVTIPCKET QNTVCNCHAG FFLRESECVP CSHCKKNEEC MKLCLPPPLA NVTNPQDSGT A

相关搜索：重组小鼠sTNF RI/TNFRSF1A，sTNF RI/TNFRSF1A, Murine